作　　者： ; ; ; ; ;

机构地区： 华南农业大学资源环境学院植物病理学系

出　　处： 《华南农业大学学报》 2006年第2期39-41,共3页

摘　　要： 对离体筛选的水稻抗纹枯病突变体(突变型)和未经筛选的原品种(原始型)的愈伤组织经水稻纹枯病菌Rhizoctonia solani粗毒素处理后24 h内的苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和多酚氧化酶(PPO)活性以及木质素含量的变化进行了研究.结果表明:突变型的PAL、POD和PPO比活性以及木质素含量均明显高于原始型;8 h时,PAL、POD、PPO比活性和木质素含量(D280 nm)在突变型和原始型中分别为5.493 1,0.022 0、24.896 6、2.924 2 U.mg-1.m in-1和2.716 5、0.009 9、18.838 7、1.732 1;上述3种酶活性在突变型中出现波峰的时间一般在8 h,早于原始型的12 h或更迟.由此说明离体筛选的水稻抗纹枯病突变体的抗病机制与一般抗性品种类似. Changes in activities of phenylalanine ammonialyase （ PAL）, peroxidase （POD） and polyphenol oxidase （PPO）, and contents of lignin in mutant and original rice calli after treated with crude toxin of rice sheath blight pathogen Rhizoctonia solani were investigated in 24 h. The results showed that the activities of PAL, POD and PPO, and contents of lignin in mutant calli were obviously higher than those in original calli; the activities of PAL, POD and PPO, and contents （D280nm） of lignin in mutant and original calli in 8 h were 5. 493 1, 0. 022 0, 24. 896 6, 2. 924 2 U·mg^-1·mn^-1 and 2. 716 5, 0.009 9, 18. 838 7, 1. 732 1, respectively. Furthermore, activity peaks of the above-mentioned three defensive enzymes appeared in mutant calli in 8 h earlier than those appeared in original calli in 12 h or later. These results suggested that in vitro screened rice somatic mutants resistant to sheath blight had the similar resistant mechanism as general resistant varieties.

关 键 词： 水稻 抗病突变体 生理生化特征 水稻纹枯病菌 病原菌毒素

领　　域： [农业科学] [农业科学]