作　　者： ; ; ; ; ; ;

机构地区： 南方医科大学

出　　处： 《神经解剖学杂志》 2006年第2期207-213,共7页

摘　　要： 为探讨大鼠主动逃避学习后转录因子pElk1在脑内表达分布的时程变化,将55只成年SpragueDawley大鼠,分为正常对照组、Y迷宫训练组和假训练组,其中训练组与假训练组再各分为训练后0、1、3、6、24h组,每组动物各5只。训练组动物接受Y迷宫光-电结合训练,假训练组动物接受光电不结合假训练。应用免疫组织化学方法检测各组大鼠脑内各区pElk1分布及表达的变化。结果发现:pElk1免疫阳性神经元在全脑内分布广泛,在纹状体边缘区皮层大部、下丘脑、杏仁核、海马、尾壳核、边缘区、小脑均有较强表达;Y迷宫训练后0、1、3、6h,在海马、皮层大部、杏仁核、下丘脑、纹状体尾壳核及边缘区、小脑均有pElk1免疫阳性神经元表达的持续增强,训练后24hpElk1免疫阳性反应回归到正常组水平;假训练组在假训练后各时间点也有皮层大部、杏仁核等部位的表达增强,但在海马、尾壳核、纹状体边缘区等部位表达增强不明显,与训练组表达强度相比,差异有显著性。以上结果表明:pElk1在全脑分布广泛,Y迷宫学习增强海马、尾壳核、纹状体边缘区等区域的pElk1的表达,提示pElk1可能参与了Y迷宫相关的各脑区的学习记忆活动。 To investigate the distribution of pElk-1 in rat brain and the temporal expression change after electrical Y-maze active avoidance learning, 55 adult Sprague-Dawley rats were divided into normal group, Y-maze training group, pseudo-training group. The latter two groups were divided into 0 h, 1 h, 3 h, 6 h, 24 h after training subgroups. The Y-maze training group animals received light-electricity integrated training and the pseudo-training group animals received light-electricity non-integrated training, pElk-1 expression was detected with immunohistochemical method, pElk-1 immuno-positive neurons distributed diffusely in rat brain, pElk-1 had intensive expression in hippocampus, cortex, hypothalamus amygdala, eaudate putamen, marhinal division of striatum （MrD） , cerebellum, In the Y-maze training groups, the pElk-1 expression level increased immediately after the training in hippocampus, cortex, amygdala, hypothalamus caudate putamen, MrD, cerebellum, and maintained in 1 h, 3 h, 6 h after training. 24 h after the training, pElk-1 expression returned to normal level in all groups. The pseudo-training groups had increased pElk-1 expression in cortex and amygdala at 0 h, 1 h, 3 h, 6 h after pseudotraining too. But compared with the training groups, the expression increase in hippocampus, caudate putamen and MrD was slight in the pseudo-training groups and these differences were statisticaly significant, pElk-1 has widespread distribution in rat brain, Y-maze learning potentializes pElk-1 expression in hippocampus, caudate putamen and MrD. The present results suggest that the activation of pElk-1 may contribute to Y-maze-related learning and memory activities in different brain areas.

关 键 词： 学习与记忆 脑 大鼠

领　　域： [生物学] [生物学] [建筑科学]