作　　者： ; ; ;

机构地区： 宁波大学理学院

出　　处： 《宁波大学学报（理工版）》 2006年第1期18-22,共5页

摘　　要： 在pH3．2的B—R缓冲溶液中，研究了苯胺红T（ST）与核酸相互作用的共振瑞利散射（RRS）光谱及其分析应用．此时，ST主要以一价阳离子HR^＋形式存在，核酸以聚阴离子[（NA）^n-]形式存在．大量的阳离子染料通过静电引力和疏水作用力聚集在核酸表面形成超分子化合物，从而导致RRS强度急剧增强，产生的RRS光谱锐利，实验的灵敏度显著提高，检测范围加宽0～8．0μg／mL，检测限达4、0ng／mL，可用于痕量DNA样品分析．考察了某些共存物质的影响，并用于合成样品和实际样品中核酸的测定，获得满意的结果．利用Scatchard方程研究了染料和核酸之间的结合个数和结合常数分别为14和1．6×10^4L／mol. In pH 3.2 B - R acidic buffer medium, the investigation is conducted on resonance rayleigh scattering （RRS） of the safranine T （ST）-nucleic acids system and its analytical application. In the experiment, ST exist as a HR ＋ and Nucleic acids as [ （ NA）^n- ], and they can react on each other easily to form ST nucleic supermolecule as a result of static gravitation and hydrophobic interaction. The signal of RRS spectrum of the complex is enhanced sharply and obviously. Its enhancement is in direct proportion to the concentration of nucleic acids in the range 0 - 8.0 μg/mL for calf thymus DNA , and the corresponding detection limits is 4.0 ng/mL for calf thymus DNA. The method is applied to the determination of trace amounts of nucleic acid in synthetic samples and natural samples, and the results turn out to be satisfactory. The binding number and intrinsic binding constant are 14 and 1.6×10^4L/mol respectively according to the Scatchard function.

关 键 词： 苯胺红 共振瑞利散射 核酸 结合数 结合常数

领　　域： [理学] [理学]