作　　者： ; ; ; ; ; ;

机构地区： 山东轻工业学院

出　　处： 《中国造纸学报》 2006年第1期25-28,共4页

摘　　要： 从碱性土壤中分离得到一株耐碱性β-1，4-聚糖酶高产细菌A-30，经初步鉴定属短小芽孢杆菌（Bacillus pumilus）。采用麸皮为主要碳源，尿素为主要氮源，在pH值8．0、温度32℃，发酵至一定时间，最高木聚糖酶活可达400IU／mL，纤维紊酶活可达0．60IU／mL以上。探讨了用其所产木聚糖酶处理漂白麦草浆以改善其性能的可能性。结果表明，A-30粗酶液可以改善草浆的滤水性、强度和脆性，最适宜的酶用量为2—5IU／g浆。 A strain A-30 which produced extracellular alkali-tolerant β-glycanase was screened and identified as Baccillus pumilus. When A-30 was cultured on complex medium containing wheat bran as main carbon source and urea as nitrogen source for a period with initial pH 8.0 and 32℃, the highest xylanase activity reached about 400 IU/mL and cellulase activity over 0.60 IU/mL. Enzymatic modification of the bleached wheat straw pulp with the crude enzymes was investigated. The results showed that the properties such as drainage, physical strength and fragility of the pulp were improved at the optimum enzyme dosage of 2 - 5 IU/g pulp.

关 键 词： 芽孢杆菌 木聚糖酶 纤维素酶 麦草浆 酶法改性

领　　域： [轻工技术与工程] [生物学]