作　　者： ; ; ; ; ;

机构地区： 华南农业大学园艺学院园艺生物技术研究所

出　　处： 《福建农林大学学报（自然科学版）》 2006年第2期173-176,共4页

摘　　要： 分析植物花分生组织特征基因APETALA1(AP1)同源基因的保守区序列,设计特异引物;用PCR方法从枇杷栽培品种香钟11号和野生种栎叶枇杷基因组DNA中各扩增出1个350 bp左右的片段;将该片段分别克隆到pUCm-T载体.测序和序列分析结果表明获得了枇杷AP1同源基因的片段.该基因片段的序列在2个不同种的枇杷间差异较小,均含有2个内含子,特别是外显子部分只有1个碱基的差别;编码区共编码36个氨基酸,氨基酸序列也只有1个氨基酸的差异.其序列已经在GenBank中登记(登录号分别为AY549306和AY571786).同源性比较发现该基因片段与其他作物中已经报道的AP1同源基因的同源性大都在80%以上,特别是与同属于蔷薇科的苹果的同源性最高,达到91%(栽培种)和94%(野生种),推测它们具有相似的功能. A pair of primers were designed according to the conservative regions of the plant floral meristem identity gene-- APETALA1 （API） homologous genes. In both loquat cultivar Xiangzhong 11 and wild variety Liye, a fragment about 350 bp of AP1 homologous gene was amplified respectively by polymerase chain reaction （PCR） using the gonomic DNA. The fragments were cloned into pUCm-T vector, and then sequenced. The results showed that a fragment of AP1 homologous gene was obtained ; the fragments from the two varieties were named with ejAPI and epAP1 accordingly. The results of sequence analysis indicated that epAP1 and ejAP1 had little difference, there were two intruns in the fragment of beth varieties of loquat, and the exons were most similar with each other, there was a difference in one nucleotide acid between epAP1 and ejAP1. They encoded 36 amino acids. The two genes were registered in GenBank with the the accession numbers of AY549306, AY571786 accordingly. After the deduced amino acid sequences of the fragments were submitted to GenBank and blasted with API homologous genes of other plants, it was found that the homology with most of the other API homologous genes reached 80%, especially to Malus family-apple, the homology reached 91% （cultivar） and 94% （wild） , to the highest level. This result suggested that ejAP1 and epAPl genes might have the same function as other API homologous genes.

关 键 词： 枇杷 基因 分子克隆 序列分析

领　　域： [农业科学] [农业科学] [生物学]