作　　者： ; ; ; ; ;

机构地区： 广西大学生命科学与技术学院

出　　处： 《工业微生物》 2006年第1期43-46,共4页

摘　　要： 经过初筛、复筛,从活性污泥中分离出一株富产絮凝剂并适用于甘蔗蔗汁絮凝澄清的微生物菌株A。根据生化和生理特征及光学显微镜和电镜扫描的形态观察,初步鉴定该菌株为轮枝孢属(Verticilliumsp.)。同时对菌株进行培养条件的初步实验,获得该菌株产絮凝剂的最佳培养条件为:pH6.0,28℃,140r/min,培养4d。最佳碳氮源分别为葡萄糖和牛肉膏。微生物发酵液在蔗汁沉降试验中的絮凝率为69.2%,清汁色值IU为87.4。 By means of primary screening and double-screening, a strain A producing rich flocculation was obtained from activated sludge, The strain was identified as Verticillium alboatrum by the physiological and biochemical properties the colony form observed with optical microscope and electrical scanning photomicrograph. Optimization of culture conditions for the strain was investigated. The optimal fermentation conditions were obtained as following： pH6.0, 28℃, 140r/min, 4d. The best carbon and nitrogen source were glucose and beef extract respectively. In the treatment of sugarcane juice broth from Verticillium alboatrum fermentation, the flocculation rate of 69.2 %, A560 of 0. 201 and colourity of 87.4IU were obtained.

关 键 词： 微生物絮凝剂 轮枝孢属 培养条件 絮凝 色值

领　　域： [轻工技术与工程] [轻工技术与工程] [环境科学与工程]