作　　者： ; ; ; ;

机构地区： 西北农林科技大学食品科学与工程学院

出　　处： 《食品研究与开发》 2006年第2期12-14,17,共4页

摘　　要： 以壳聚糖膜为载体,戊二醛为交联剂制备固定化乳糖酶,筛选酶固定化的最优条件。结果显示:戊二醛浓度为2.5%,pH值为6.5,乳糖酶浓度为0.4mg/mL,交联时间12h,固定化12h时,获得了最佳的固定化效果。 β-Galactosidase was immobilized on chitosan membran crosslinked with glutaraldehyde.The immobilization conditions of immbilization enzyme was investigated in this experiment.The best efficiency of immobilization could be obtained under the condition that the concentrantion of glutaraldehyde was 2.5 %, the pH of glutaraldehyde was 6.5, the adding amount 13-Galactosidase was 0.4 mg/ mLerosslink and immobilization time both were 12 hours.

关 键 词： 乳糖酶 固定化 壳聚糖膜 戊二醛

领　　域： [轻工技术与工程] [化学工程] [生物学]