作　　者： ; ; ; ; ; ; ; ;

机构地区： 江西农业大学动物科学技术学院动物生物技术国家重点实验室培育基地

出　　处： 《Acta Genetica Sinica》 2006年第3期213-219,共7页

摘　　要： 前列腺素内过氧化物酶2是花生四烯酸合成前列腺素的限速酶,在包括排卵、受精、着床、分娩等一系列生殖过程中起着重要作用,因而编码该酶的基因是影响繁殖性状的重要候选基因。通过PCR-RFLP分析前列腺素内过氧化物酶2 基因在15个中外不同繁殖性能猪种中的遗传变异,结果表明,不同类型中国地方猪种和外来商业猪种在此基因位点上存在丰富的多态性,繁殖性能相对较好的江海型、华北型和华中型猪种中A等位基因表现为优势等位基因,卡方检验显示其基因频率分布与西方商业猪种及繁殖性能较低的高原型藏猪和华南型猪种差异均极为显著(P<0．001)。利用二花脸×杜洛克资源家系F2群体分析该基因与繁殖性状的相关性,在180头F2代母猪群体中,未能进一步证实该基因位点对总产仔数、产活仔数和死胎数3个繁殖性状存在显著影响(P=>0．05),但携带优势等位基因A的个体趋向于拥有较高的总产仔数、产活仔数和偏低的死胎数,鉴于该基因的重要作用,基于全基因序列的SNP扫捕和大样本群体的相关性分析仍很有必要。 Identification of major genes that genetically impact female fertility is important for successful selection of high prolificacy pig lines. Because it is the rate-limiting enzyme in the conversion of arachidonic acid to prostaglandins （PGs）, which are important for ovulation, fertilization, implantation, decidualization and parturition, prostaglandin-endoperoxide synthase 2 gene （PTGS2） is a potential candidate gene affecting porcine reproductive traits. In this study, a PCR-RFLP was used to genotype a total of 1 031 animals, including 661 from twelve Chinese local pig breeds, 190 from three Western pig breeds and 180 F2 sows from Nanchang pig resource family. Differences in frequency distributions of PTGS2 among twelve Chinese and three Western pig breeds and populations generally agree with their prolificacy. The allele frequencies in Lower Changjiang River Basin Type pig breeds, North China Type and Central China Type breeds are significantly different from those in South China Type, Plateau Type and Western pig breeds （P〈0.001）. And no significant differences were observed among Lower Changjiang River Basin Type, North China Type, Central China Type pig breeds, between South China Type and Western pig breeds, in part because of similar fertility patterns. And notable associations as well as reliable additive and dominant effects were not detected in an Erhualian × Duroc F2 population （P〉0.05）. Whereas, there is a trend for animals with one copy of the favourable A allele to have an increased TNB （total number of piglet born） and TBA （the number of piglets born alive） and a decreased SB （stillborn pigs） trait. Considering its crucial role in reproductive pathways, the PTGS2 gene deserves further study.

关 键 词： 猪 繁殖性状

领　　域： [生物学] [农业科学] [农业科学]