作　　者： ; ; ; ; ;

机构地区： 中国农业大学农学与生物技术学院

出　　处： 《植物病理学报》 2006年第1期86-90,共5页

摘　　要： 比较了Calcofluor、苯胺蓝、荧光素钠3种荧光染色方法和曲利苯兰酒精-乳酚酸、考马斯亮兰和苯胺蓝组织透明染色法对黄瓜霜霉病菌不同生长发育阶段的染色效果。结果表明,在一定的染液浓度和pH值条件下,3种荧光染色方法均可进行孢子囊、休止孢、芽管、附着胞、孢囊梗等寄主组织外部菌体的原位染色,其中Calcofluor染色方法更稳定、简捷和易于观察。曲利苯兰组织透明染色法能观察黄瓜霜霉病菌的整个生长发育阶段,但该法更适宜胞间菌丝及吸器的染色。 Fluorescence staining methods of calcofluor, aniline blue, and uranine and the techniques of alcoholic lactophenol trypan blue, coomassie brilliant blue and aniline blue for different phases in life cycle of Pseudoperonospora cubensis were comparaed. The results showed that three kinds of fluorescence techniques can be used to stain sporangia, cystospore, germ tube, appressorium, and sporangiophore of P. cubensis in situ on the leaf surface. But staining with calcofluor was easier to handle, faster, and more reliable than the other two. Trypan blue was appropriate for staining of the intercellular hypha and haustorium.

关 键 词： 荧光染色 组织学观察 真菌原位检测

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]