作　　者： ; ;

机构地区： 安徽农业大学茶与食品科技学院茶叶生物化学与生物技术教育部农业部重点实验室

出　　处： 《食品与生物技术学报》 2006年第1期40-44,共5页

摘　　要： 比较了海藻酸钠和壳聚糖两种载体及不同固定化方法对单宁酶和β-葡萄糖苷酶的共固定化效果,结果表明以海藻酸钠为载体,采用交联-包埋-交联固定化方法的效果最佳。对共固定化条件进行了优化,可使单宁酶和β-葡萄糖苷酶的活力回收率分别达67.3%和46.0%。 The effects of two kinds of carriers and four different kinds of immobilized methods on the co-immobilization of tannase and β-glucosidase were investigated in this work. The results showed that the best co-immobilization could be obtained by selecting sodium alginate as the immobilized carrier and using the crosslinking-embedding-crosslinking method. The conditions of co-immobilization were also optimized, under which the activity recoveries of tannase and β- glucosidase could reach 67.3 % and 46.0%, respectively.

关 键 词： 单宁酶 葡萄糖苷酶 海藻酸钠 壳聚糖 共固定化

领　　域： [生物学]