作　　者： ; ; ; ; ; ; ; ;

机构地区： 吉林大学白求恩医学院

出　　处： 《中国兽医学报》 2006年第1期9-10,27,共3页

摘　　要： 以Chap10-VP1融合蛋白为包被抗原,建立了检测口蹄疫病毒特异性抗体的间接ELISA方法。抗原包被浓度为50μg/L时,血清最佳稀释度为1∶400,病毒与阳性血清的反应可被Chap10-VP1蛋白阻断,检测的灵敏度为1∶1600。 Using Chap10 VP1 fusion protein which was constructed and expressed in our lab as coating antigen to replace FMDV.an indirect ELISA method was established by detecting the anti-VP1 antibodies in the serum from Guinea pig immunized with the inactivated vaccine of type O of FMDV. For the indirect ELISA method.the optimal concentration of coating antigen was 50 mg/L with the optimal serum dilution of 1 ： 400. In this condition.the ,specific anti-VP1 antibodies eouId be detected. The Chap10-VP1 fusion protein could block the response between FMDV and positive serum. The sensitivity of the indirect ELISA was 1 ： 1 600.

关 键 词： 重组蛋白 间接 口蹄疫病毒抗体

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]