作　　者： ; ;

机构地区： 福建农林大学园艺学院亚热带果树研究所

出　　处： 《福建农业大学学报》 1996年第3期302-306,共5页

摘　　要： 以紫果西番莲的腋芽萌发的芽苗为基础试材，以顶芽、茎节间和叶柄作为外植体，培养在改良ＭＳ培养基（大量元素减半）附加ＩＢＡ１—４ｍｇＬ－１、蔗糖２％的培养基上诱导生根，在ＩＢＡ２和４ｍｇＬ－１的条件下，顶芽生根率均为１００％，在ＩＢＡ２ｍｇＬ－１的条件下，茎段和叶柄的生根率达８０％；茎节间、根段和叶柄作为外植体，培养在改良ＭＳ培养基（大量元素减半）附加ＺＥＡ２、２．５或３ｍｇＬ－１，蔗糖２％的培养基上诱导不定芽，在ＺＥＡ２，２．５和３ｍｇＬ－１的条件下，茎段的长芽率均为１００％，在ＺＥＡ２．５ｍｇＬ－１的条件下，根段和叶柄的长芽率分别为３３％和３７％ Shoots of Passiflora edulis germinated from axillary buds were used as experimental material for morphogenesis in vitro. Terminal bud, internode segment and leaf stalk of P. edulis, all of which were cut from the shoots, were cultured on modified MS medium (macroelements being half strength) supplemented with 2% sucrose and 1-4 mg·L -1 IBA. The most effective rooting treatment was achieved with terminal buds cultured in 2 or 4 mg·L -1 IBA, the induction rate of rooting being as high as 100%, and rooting occurred in as early as 10-12 d; while the rooting rate both internode segment and leaf stalk cultured in 2 mg·L -1 IBA were 80%. Internode segment, root segment and leaf stalk explants were cultured in modified MS basal medium (macroelements being half strength) added with 2% sucrose and 2-3 mg·L -1 ZEA, the most effective budding treatment was achieved with internode segment, the induction rates of the shoots was 100% in either 2, 2.5 or 3 mg/L ZEA,33% and 37% of induction rate of the shoots were attained with root segment and leaf stalk in 2.5 mg·L -1 ZEA, respectively, higher than that in 2 or 3 mg·L -1 ZEA.

关 键 词： 紫果西番莲 西番莲 形态发生 顶芽 茎节间 叶柄

领　　域： [农业科学] [农业科学]