作　　者： ; ; ; ; ; ;

机构地区： 韶关学院英东生物工程学院

出　　处： 《韶关学院学报》 2005年第12期74-77,共4页

摘　　要： 报道了双青35号菜豆不同外植体在含有不同激素的培养基上诱导、分化及生根情况,并建立其高效再生体系.研究结果表明:愈伤组织诱导和增殖培养基为MS+6-BA2.0 mg/L+NAA2.0 mg/L,不定芽再生培养基为MS+6-BA2.0 mg/L+NAA0.2 mg/L,生根培养基为1/2MS+IAB3.0 mg/L+0.1%活性炭是较佳的. Differentiation and rooting of different explants of Phaseolus vulgaris cv. “Shuangqing 35” were described on different medium in which contained different hormones and a high efficient regeneration system was established. The result showed that the most suitable medium of inducing and proliferating callus is MS ＋ 6 - BA 2.0 mg/L ＋ NAA 2.0 mg/L, medium of regenerating adventitious bud is MS ＋ 6 - BA 2.0 mg/L ＋ NAA0.2 mg/L, rooting medium is 1/2MS ＋ IAB3. 0 mg/L ＋ 0.1% mg/L active carbon.

关 键 词： 双青 号菜豆 不同外植体 再生体系

领　　域： [生物学]