机构地区: 华南农业大学农学院广东省植物分子育种重点实验室
出 处: 《华南农业大学学报》 2006年第1期1-4,共4页
摘 要: 在筛选和鉴定水稻T-DNA(D s)插入纯合体的过程中,观察到1个叶片卷曲的突变体.对该突变体进行遗传分析表明,分离群体出现叶片卷曲和叶片正常2种植株类型,其分离比率为3∶1,符合1对基因的显性遗传.Basta抗性检测及PCR分子检测证实,该突变体是由单一T-DNA(D s)插入所引起的,突变性状与T-DNA(D s)共分离.该突变材料可用于插入座位的基因克隆. A roiled leaf mutant, Oryza sativa L. subsp, japonica IL39, caused by T-DNA (Ds) insertion was identified. Genetic analysis of the mutant showed that the two types of phenotype, rolled leaf and normal leaf in the segregating populations derived from the T-DNA (Ds) heterozygotes, fit the ratio of 3: 1. Test for Basta resistance showed that the rolled leaf plants were all resistant while the normal leaf plants were susceptible. The ratio of resistant and susceptible plants is 3:1 , which indicate that the rolled leaf mutant is co-segregated with Basta resistance. The rolled leaf mutant caused by T-DNA (Ds) insertion was further confirmed by T-DNA detection using PCR method. This rolled leaf mutant is useful for isolation of the tagged gene in rice,
领 域: [生物学]