作　　者： ; ; ; ;

机构地区： 南方医科大学

出　　处： 《广东微量元素科学》 2005年第11期49-54,共6页

摘　　要： 运用微波消化系统处理生物样品,以 2,3-二氨基萘为荧光试剂建立了一种测定生物样品中 Se 的荧光分光光度法.方法的最低检出限为 1.6 ng/mL,线性范围 0～0.600 μg/mL,回收率 92.22%～99.78%,连续10次测定样品变异系数(CV)为0.4%(n＝10),隔2日重复实验变异系数为 1.54%.该法具有灵敏、稳定、可靠等优点,适用于生物样品中 Se 含量的测定. Biologic samples were digested by a system of micro wave, a method of fluorometric determination of selenium was established, the selenium of which brought out a new compound with 2, 3-diaminonphthalene. The sensitivity was 1.6 ng/mL and the linearity was 0 ～0.600 μg/mL. The recoveries ranger was from 92.22% to 99.78%. The CV of instrtunent was 0.40% （ n = 10） and the CV of repeated relation was 1.54 %. This method is sensitive, accurate and suitable for the routine analysis of selenium in biologic sampie.

关 键 词： 硒 荧光分光光度法 二氨基萘

领　　域： [理学] [理学]