作　　者： ; ; ;

机构地区： 中国科学院

出　　处： 《武汉植物学研究》 2005年第6期514-518,共5页

摘　　要： 通过用一对特异性引物,PCR扩增得到花生(A rach is hyp og aea)主要致敏蛋白A ra h 1的基因的启动子片段1 957 bp,其序列组成与已发表序列基本一致。用其替换pB IN 35S-mGFP 4载体中的35S启动子部分,组成一种花生转基因表达载体pGA 1。该载体含有种子特异表达调控元件、增强表达元件和一些与转录因子结合的调控元件,是一个强启动子,便于将外源基因转入花生,实现外源基因在花生中的表达,为建立花生生物反应器奠定了基础。 The promoter of the gene encoding the major peanut allergy protein Ara h I has been cloned by the polymerase chain reaction technique（PCR） with a pair of special primers. The promoter we have got is 1957 bp. Basically,it is consistent with the sequence published. By replacing the 35S promoter in pBIN 35S-mGFP4 vector with this promoter,we have constructed a plant transgenic expression vector of peanut named pGA1. It contains seed specifically expressed ele- ments, enhancers and some elements regulated by transcription factors. Thus the heterologous gene can be transferred to peanut and expresses in it easily. The research lays a foundation for establishing the peanut bioreactor.

关 键 词： 启动子 表达载体 酶切

领　　域： [生物学]