作　　者： ; ; ; ; ; ;

机构地区： 广东药学院基础学院

出　　处： 《广东药学院学报》 2005年第6期709-711,共3页

摘　　要： 目的我国汉族人群FcαR1基因5-UTR区3个SNPs多态性颁的特征及将其与日本人群进行比较。方法提取368例正常广东汉族人基因组,DNA扩增FcαR1基因5-UTR相关区域并直接测序鉴定基因型。结果①我国汉族人群FcαR1基因5-UTR区3个SNPs多态性:-114位点TT基因型最常见(52.7%),CC基因型分布最少(7.3%),-27位点TT(85.1),CC(1.4%),+56位点TC基因型最常见(51.4%),CC基因型分布最少(12.0%);②等位基因分布频率:-114位点T(72.7%)、C(27.3%);+56位点T(62.4%)、C(37.6%)及-27位点T(91.8%)、C(8.2%);③主要单倍型:TTT(61.9%)、CTC(27.2%)和TCC(7.9%)。结论与日本人群比较,我国汉族人群FcαR1基因5-UTR区-114T/C及+56T/C无论是基因型,还是等位基因频率分布,差异均无显著性。 Objective To investigate the distribution of SNPs in FcαR1 gene 5＇-UTR and promoter region and to compare them with the Japanese. Methods Genomic DNA was extracted from 368 healthy Chinese Han subjects, genotypes of the SNPs in FcαR1 gene 5＇ - UTR and promoter region were determined by PCR and direct sequencing. Comparing between two groups was conducted with chi square test. Results 1. the genotypes＇ frequencies of the 3 SNPs in FcαR1 gene 5＇ - UTR and promoter region were Tr （52.7%） ,CC （7.3%） in - 114 locus,TT（85.1% ） ,CC（ 1.4% ） in - 27 locus, TC （51.4%） ,CC （ 12.0% ） in ＋56 locus. 2. the alleles＇ frequencies of the 3 SNPs in FcαR1 gene 5＇ - UTR region were T（ 72.7% ） , C （27.3%） in - 114 locus; T（62.4% ）, C （37.6%） in ＋ 56 locus and T （91.8%）, C（8.2% ） in -27 locus. 3. Main haplotype pattern is TIT （61.9%）, CTC（27.2% ） and TCC （7, 9% ）s. Conclusion There was no significant difference in the distribution of SNPs in FcαR1 gene 5＇ - UTR and promoter region in Chinese Han compared with the Japanese.

关 键 词： 受体 单核苷酸多态性 连锁不平衡 单倍型

领　　域： [生物学]