作　　者： ; ; ; ; ;

机构地区： 中国科学院华南植物园

出　　处： 《吉首大学学报（自然科学版）》 2005年第2期18-23,共6页

摘　　要： 用焦锑酸钾沉淀法研究了包台型不育水稻及其保持系花药发育早期细胞中的Ca2+分布变化.从花粉母细胞时期开始,不育系与保持系花药中的Ca2+分布出现差异,不育系药壁外层和药室内壁及药隔中均有Ca2+出现,而保持系花药中基本没有Ca2+分布,在减数分裂时期表现更加明显,不育系药壁和药隔中的Ca2+相对于花粉母细胞时期明显地有所增加,特别是绒毡层细胞和药隔中木质部细胞的次生加厚壁上Ca2+增加尤其明显,而保持系花药中的Ca2+较少.笔者从花药发育的更早期去寻找水稻雄性不育的机理. Potassium pyroantimonate technique was used to investigate the Ca^2＋ distribution in the anthers of Baotai type cytoplasmic male-sterile rice （Ozyra sativa L. ） and its maintainer line. There were some differences between sterile and fertile anthers during the pollen mother cell stage. Some Ca^2＋ were found in epidermal cells, endothecium and vascular bundle of the sterile rice, whereas almost no Ca^2＋ in fertile anthers. The differences were further improved at the meiosis stage. Ca^2＋ in cells of sterile anthers＇ wall and vascular bundle apparently increased, especially at tapetal cells and the second wall of trachea. It was manifested that at the early stage of anther＇s development, gene expression and metabolism of fertility in the sterile and fertile anthers appeared differences, which caused the pollen abortion afterwards. The phenomena offered a new ponder for us on the male sterile mechanism research of the rice. In other words, we should seek the mechanism of rice male sterile at the early development of anther.

关 键 词： 水稻 雄性不育 焦锑酸钾沉淀法 花药

领　　域： [生物学]