作　　者： ; ; ;

机构地区： 华南农业大学食品学院

出　　处： 《安全与环境学报》 2005年第6期26-29,共4页

摘　　要： 从自然界中筛选出6株相互间无拮抗作用的纤维素分解菌进行混合发酵,研究了该混菌以工业废弃物木薯渣为原料发酵的最佳产酶条件是:培养基配方为木薯渣4 g,黄豆粉0.75 g,KH2PO40.20 g,NaCl 0.02 g,MgSO4.7H2O 0.015 g,蒸馏水1 000 mL;发酵工艺为初始pH=7.0,培养温度40℃,每250 mL锥形瓶装培养液140 mL,接种量3%,在140 r.min-1转速下发酵72 h。结果表明,混合菌的发酵可大幅度提高纤维素酶活力及对还原糖的耐受力。 The present paper intends to introduce the authors＇ research on the best cultivation conditions of the mixed cellulolytic strains to produce cellulase. In doing so, the six cellulolytic strains were screened out from nature, which should be isolated with no mutual amensalism. And then the strains were let to be co-fermented. The optimum conditions for co-fermentation prove to be as follows： cassava residue 4 g, soybean powder 0.75 g, KH2PO4 0.20 g, NaCl 0.02 g, MgSO4·7H2O 0.015 g; initial pH=7.0, temperature 40 ℃, inocula 3% （φ）, and the multistrains were grown in 250 mL tack containing 140 mL of nutrient solution. Furthermore, the enzyme activity was then enacted by the coermentation. After cultivating 72 hours, the highest CMC-ase activity would be 4 731.8 μg·30 min^-1· mL^-1 wit the concentration of deoxidize glucide being 5 012.2μg·mL^-1. Using waste cassava residue helps to produce cellulase that could solve the pollution of waste and increase the value of the corresponding raw materials.

关 键 词： 发酵工程 纤维素分解菌 混菌发酵 废物利用 木薯渣

领　　域： [轻工技术与工程] [化学工程]