作　　者： ; ; ; ;

机构地区： 华南理工大学食品与生物工程学院

出　　处： 《中国生物工程杂志》 2005年第B04期235-238,共4页

摘　　要： 目的:探索利用酿酒酵母系统表达乙型肝炎病毒(HBV)preS／S基因。方法:利用PCR技 术,以HBV病毒DNA为模板,体外扩增HBV preS／S基因。然后构建重组表达载体pESC-preS／S。 用LiAc法转化酿酒酵母YPH50,选取重组菌进行培养,并诱导表达外源蛋白。提取蛋白浓缩后 进行SDS-PAGE分析,并经Western blot分析鉴定。结果:实验结果表明重组菌能够表达HBV preS／S蛋白。结论:利用酿酒酵母系统可成功表达HBV preS／S基因,为制备新型预防性疫苗提供 条件。 Objective： To explore the HBV preS/S expression in the S, cerevisiae system. Method： HBV preS/S gene fragments were amplified from HBV DNA in vitro, using PCR techniques. The amplified preS/S gene fragments were incorporated into the expression vector pESC to construct the pESC-preS/S. LiAc method were used to transfer this construct into the strain of S, cerevisiae. Protein synthesis after induction was analyzed with SDS-PAGE and Western blot. Results： It was demonstrated that the rec.ombinant yeast could express HBV preS/S protein. Conclusion： the S. cerevisiae system have been used successfully in our experiments to express the HBV preS/S gene, which provide a basis for further research and development of new HBV vaccine for hepatitis infections.

关 键 词： 基因 酵母表达系统 蛋白表达

领　　域： [生物学] [轻工技术与工程] [轻工技术与工程]