作　　者： ; ; ;

机构地区： 中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室

出　　处： 《云南植物研究》 2005年第6期669-675,共7页

摘　　要： 将口蹄疫病毒外壳蛋白VP1基因克隆到植物表达载体pBI121,并转化到根癌农杆菌(Agrobacteriumtumefaciens)菌株LBA4404中,采用叶盘转化法转化柱花草(Stylosanthesspp.)栽培品种热研二号柱花草(S.guianensiscv.ReyanⅡ),获得了转基因植株,经PCR、PCR-Southern blot和Southern blot分析表明VP1基因已整合到转基因柱花草植株的核基因组中。经RT-PCR、Northern blot分析表明VP1基因已在转基因柱花草中获得转录。 Tender leaves of Stylosanthes guianensis cv. Reyan Ⅱ were transformed with Agrobacterium tumefaciens LBA4404 carrying the gene VP1 coding food-and-mouth disease virus outer capsid protein. After 3 times kanamycin-resistant selection, the regenerated plantlets were analyzed further by PCR, PCR- Southern blot and Southern blot, the results confirmed that the VP1 gene were integrated in the genome. The results of RT-PCR and Northem blot of transgenic plants showed that VP1 gene was transcribed in transgenic plants.

关 键 词： 口蹄疫病毒 柱花草 基因 遗传转化

领　　域： [生物学]