作　　者： ; ; ; ; ; ; ;

机构地区： 广东省中医院

出　　处： 《中国中医骨伤科杂志》 2005年第6期15-18,共4页

摘　　要： 目的:探讨参麦液对软骨细胞增殖与DNA合成的影响。方法:运用关节软骨体外培养方法,设立空白对照与IGF-Ⅰ药物对照组,采用M TT比色法与二苯胺显色法观察不同浓度参麦液对软骨细胞增殖与DNA合成情况。结果:参麦液与生长因子IGF-Ⅰ均可促进软骨细胞增殖与DNA合成,同空白对照组相比具有非常显著性差异(P<0.01),其中5%参麦液组同浓度为5 ng/m l的IGF-Ⅰ组作用效果相当(P>0.05)。结论:参麦液可促进软骨细胞的合成代谢,减少其分解代谢,从而减轻关节软骨的退变,延缓OA的进展。 Objective：To study the effect of shenmai liquid on the proliferation and DNA synthesis of chondrocyte. Methods：Joint chondroctye was cultured in vintro and randomly distributed into eight groups, that is the blank control group, the 1 percent shenmai liquid group, the 2.5 percent, the 5 percent, the 7.5 percent, the 10 percent, the IGF- Ⅰgroup（5ng/ml） and the IGF- ⅠⅡ group （10ng/ml） . MTT and diphenylamine method were adopted in the study. Results： Both shenmai liquid and IGF- Ⅰcoulcl facilitate the proliferation and DNA synthesis of chondrocyte. There was great significant difference compared with the blank group（P〈0. 1）. There was no difference between the two groups of 5 percent shenmai liquid and IGF- Ⅰ group （5ng/ml）（P〉0.05）. Conclusions： Shenmai liquid could both promote anabolism and decrease catabolism of the chondrocyte , thus it could defer the degeneration of osteoarthritis cartilage.

关 键 词： 参麦液 软骨细胞 细胞培养

领　　域： [生物学]