作　　者： ; ; ;

机构地区： 汕头大学医学院炎症与免疫性疾病研究所

出　　处： 《激光生物学报》 2005年第5期377-379,共3页

摘　　要： 目的:研究PAR-2激动剂SLIGKV和tc-LIGRLO、胰蛋白酶及其抑制剂对H292肺上皮细胞[Ca2+]i的影响。方法:应用Fluo-3/AM荧光标记技术和激光扫描共聚焦显微镜(LSCM)检测不同因素处理的H292肺上皮细胞[Ca2+]i。结果:胰蛋白酶、SLIGKVt、c-LIGRLO均能引发H292细胞[Ca2+]i的增加,平均荧光强度分别比加入药物前增加267%,60%和37%。胰蛋白酶抑制剂大豆胰蛋白酶抑制剂(SBTI)和α1-抗胰蛋白酶(α1-AT)可以抑制胰蛋白酶诱导的细胞[Ca2+]i的增加。结论:PAR-2可以介导H292肺上皮细胞[Ca2+]i的释放增加,胰蛋白酶抑制剂可以抑制胰蛋白酶诱导的细胞[Ca2+]i的增加。 Objective： To study the effects of PAR-2 agonists SLIGKV and tc-LIGRLO, trypsin and its inhibitors on intracellular calcium concentration [Ca^2＋ ]i in the H292 lung epithelial cell. Methods： Fluo-3/AM fluorescence technique and laser scanning confocal microscope （LSCM） were used to measure the intracellular calcium level after different reagents were added in Hanks of H292 cells. Results： Trypsin, SLIGKV, tc-LIGRLO could increase the intracellular calcium concentration [ Ca^2＋ ]i in H292 cells. Compared with control, the average fluorescent intensities were increased 267 %, 60%, 37 %, respectively. Trypsin inhibitors soybean trypsin inhibitor（SBTI） and α1-antitrypsin（α1-AT） could inhibit trypsin induced increase of [Ca^2＋ ]i in 14292 cells. Conclusion： Activatin of protease-activated receptor-2 induce increase of [ Ca^2＋ ]i in H292 cells. Trypsin inhibitors could inhibit trypsin induced the increase of [Ca^2＋ ]i in H292 cells.

关 键 词： 激光扫描共聚焦显微镜 荧光探针 蛋白酶激活受体 肺上皮细胞 胞浆内钙离子浓度

领　　域： [生物学]