作　　者： ; ; ; ; ; ;

机构地区： 华南农业大学资源环境学院

出　　处： 《华南农业大学学报》 2005年第4期73-76,共4页

摘　　要： 采用插入序列指纹图谱分析(IS-PCR)和16S rRNA基因全序列分析,对来源于优良牧草———糖蜜草M elinism inutifloraBeauv.内生固氮菌进行研究.结果表明,糖蜜草中分离的内生固氮菌与产脂固氮螺菌模式菌株DSM1691的插入序列指纹图谱存在着差异;来源于糖蜜草的4株菌紧密地聚在一起,与GenBank中已知产脂固氮螺菌Azospirillum lipoferum的16S rRNA基因全序列有97%的序列相似性.初步确定糖蜜草中分离的内生固氮菌为一新类群的内生固氮菌. Endophytic diazotrophs isolated from molassess grass, Melinis minutiflora , were studied by insert sequence（IS）-PCR fingerprinting patterns and full length of 16S rRNA gene sequences. IS-PCR fingerprinting showed that different patterns appeared between isolates and the type strain DSM 1691 of Azospirillum lipoferum; 16S rRNA gene sequence analyses indicated that homology between isolates and known species Azospirillum lipoferum is 97%. These resuks suggested that isolates from molasses grass are possible a new group of endophytic diazotrophs closely related to Azospirillum lipoferum.

关 键 词： 糖蜜草 内生固氮菌 固氮螺菌

领　　域： [生物学]