作　　者： ; ; ; ; ;

机构地区： 中山大学

出　　处： 《应用化学》 2005年第11期1192-1196,共5页

摘　　要： 利用溶胶-凝胶技术固定甲胎蛋白抗体,并掺入石墨粉作导体,制得可用于临床检测甲胎蛋白的电位型免疫传感器.该传感器表面可更新重复使用,响应时间40s.传感器的电位响应与甲胎蛋白的浓度呈良好的线性关系,线性范围为5.0×10-9～5.0×10-8g/mL,线性回归方程△E(mV)=3.0+0.95c(10-9g/mL),线性相关系数r=0.999 4.优化了实验条件,讨论了传感器的电位响应机理,比较了传感器表面不同的更新方法,将传感器应用于血清样品中甲胎蛋白的测定,获得满意结果. A novel immunosensor for the determination of alpha fetoprotein（AFP） based on sol-gel technique was constructed by dispersing graphite and alpha fetoprotein in tetraethylorthosilicate（TEOS） derived sol-gel. With the increase of specific immuno-reaction complex of antibody and antigen, the membrane potential of the immunosensor changes. The membrane potential value is correlative with the antigen concentration in solution. According to this relationship, AFP can be detected quantificationaUy, the linearity range for determining AFP is 5.0 × 10^-9 - 5.0 × 10^-8 g/mL with a correlation coefficient of 0. 999 4. Other antigens such as carcino emtynic antigen（ CEA）, ferritin and hepatitis B surface antigen（HBsAg） do not interfere with the determination. The dependences of the potential response on immobilization method, incubation time, sensitivity and reproducibility were studied. The immunosensor was found to be stable for about 30 days when it was stored in dry state at 4 ℃. A satisfactory determination of AFP in serum samples can be obtained by this immunosensor.

关 键 词： 溶胶 凝胶 免疫传感器 甲胎蛋白 再生

领　　域： [理学] [理学] [理学] [理学]