作　　者： ; ; ; ; ; ;

机构地区： 北京中医药大学

出　　处： 《神经解剖学杂志》 2005年第5期507-510,共4页

摘　　要： 采用MPTP腹腔注射建立Parkinson病动物模型。选用健康C57BL/6J小鼠随机分成3组:正常组、模型组和中药治疗组。动物存活期分别为14d和28d。采用ABC免疫组化和原位杂交方法对各组脑切片分别进行Bcl-2蛋白和mRNA检测。结果表明:模型组Parkinson病鼠中脑黑质内Bcl-2及其mRNA的表达在两个时段均明显低于正常组。其光密度28d比14d降低更为明显,而中药可部分上调Bcl-2及其mRNA的表达。提示:Bcl-2可能参与了Parkinson病中黑质多巴胺神经元的凋亡过程,中药可通过调节凋亡相关蛋白和基因的表达,阻止多巴胺神经元的凋亡,从而达到防治Parkinson病的目的。 C57BL/6J mice were injected intraperitoneally with MPTP to induce Parkinson＇s disease model. The experimental animals were divided randomly into three groups：（1）The normal control group （NC）;（2）The model control group（MC）;（3） Chinese medicine group（CM）;The animals were killed respectively on the 14^th or the 28^th day after first injection of MPTP. ABC immunohistochemistry and in situ hybridization techniques were used to detect the expression for Bcl-2 protein or mRNA. respectively. The results showed that the expressions for Bcl-2 and bcl-2 mRNA were less than those of normal control in substantia nigra（SN） of Parkinson＇s disease mice. The expressions were less on the 28^th day than those of the 14^th day. Reactivity for Bcl-2 and bcl-2 mRAN increased in SN of CM animals. The results suggest that bcl-2 may play an important role in apoptosis of dopaminergic neurons in PD.Chinese medicine may attenuate apoptosis of dopaminergic neurous by up-regulating the expression for Bcl-2 and bcl-2 mRAN.

关 键 词： 中约 黑质 病鼠 蛋白 中药治疗 小鼠 基因 多巴胺神经元

领　　域： [生物学] [农业科学] [农业科学] [农业科学]