作　　者： ; ; ; ; ; ;

机构地区： 中山大学化学与化学工程学院

出　　处： 《化学研究与应用》 2005年第5期623-625,共3页

摘　　要： 建立了制剂和体液中吲哚美辛的毛细管电泳高频电导分析法,并用于吲哚美辛肠溶片、复方吲哚美辛酊及血清、尿液中吲哚美辛含量的测定。对电泳介质的种类、浓度以及操作电压和进样量等影响因素进行了优化。实验采用3.0mmol/L乳酸+0.68mol/L乙醇为电泳介质,分离电压为20.0kV,可在10m in内实现对吲哚美辛的分离检测。在最佳实验条件下,吲哚美辛的线性范围为0.05μg/mL^100μg/mL,检出限为0.01μg/mL,回收率92.0%~105.1%。 A method based on capillary electrophoresis with high frequency conductivity detection was developed for determination of indomethacin in indomethacin curatorial products and body fluid. In this study, key factors for separation and determination were studied and the best analysis conditions were obtained. The buffer system of 3mmol/L lactic acid ＋ 0.68 mol/L ethanol and 20.0 kV of separation potential meet the best separation. Under the chosen condition,indomethacin in samples was eluted within 10min. The linear range of indomethacin was from 5. 0 × 10^-2 to 1.0×10^2μg/mL and the limit of detection reached 0.01μg/mL.

关 键 词： 毛细管电泳 高频电导检测 非接触式电导检测 吲哚美辛 药物 体液

领　　域： [理学]