作　　者： ; ; ;

机构地区： 中国水产科学研究院珠江水产研究所

出　　处： 《上海水产大学学报》 2005年第3期238-241,共4页

摘　　要： 为了研究感染副溶血弧菌对杂色鲍体内一氧化氮(NO)及一氧化氮合酶(NOS)活力的影响,分别对九孔鲍足部注射浓度为5×105CFU/mL、5×106CFU/mL和5×107CFU/mL的副溶血弧菌悬浮液(剂量为0.1 mm/只),在注射前及注射后6 h、12 h、24 h、48 h9、6 h1、92 h和240 h足部取血,测定其血清中NO含量以及NOS活力的变化情况。结果表明,注射副溶血弧菌后九孔鲍血清中NO含量及NOS活力显著高于对照组,且NOS活力的最大值出现的较NO最大值早,证明注射副溶血弧菌可以诱导九孔鲍血清内NO含量和NOS活力的升高。为今后研究NO/NOS系统在软体类免疫中的作用提供一些理论依据。在生产实践中,可以通过诱导调节NO在鲍体内的含量,增强其自身的非特异性免疫功能,从而提高抵抗疾病的能力。 To study the effects of infecting Vibrio parahaemolyticus on serum nitric oxide （NO） concentration and nitric oxide synthase（NOS） activity of Haliotis diversicolor supertexta, three kinds of suspension containing 5 × 10^5 CFU/mL,5 × 10^6 CFU/mL, 5 × 10^7 CFU/mL live Vibrio parahaemolyticus were injected into the foot muscle of Haliotis diversicolor supertexta （0.1 mL per abalone）respectively. The NO concentration and NOS activity in the serum were determined before injection and 6 h, 12 h, 24 h, 48 h, 96 h, 192 h and 240 h after injection. The results showed that compared to control group, injection of vibrio parahaemolyticus could induce an up-regulation of NO concentration and NOS activity. And the peak of NOS activity came earlier than the peak of NO concentration after injection. Up-regulation of NO concentration and NOS activity could be regarded as a response of hemocytes to the pathogen infection. The results of the experiment would provide some theoretical foundations for the further researches on the role which NO/NOS system plays in immunology of mollusc. In practice, in order to enhance the non-specific immunology function of abalone and strengthen its ability to resist the disease, the NO in vivo could be induced and regulated artificially.

关 键 词： 九孔鲍 一氧化氮 一氧化氮合酶 副溶血弧菌

领　　域： [农业科学] [农业科学]