作　　者： ; ; ;

机构地区： 华南农业大学食品学院

出　　处： 《食品科学》 2005年第9期494-497,共4页

摘　　要： 用克喘素(CL)与牛血清白蛋白(BSA)偶合制备的分子结合比为13.2:1的克喘素免疫原CL13.2-BSA分别免疫Balb/c小鼠、昆明鼠、SD大鼠,制备多抗血清,采用间接ELISA和间接竞争ELISA法检测,并进行多抗血清效果的比较,结果表明:昆明鼠产生多抗血清的效价和特异性均低于Balb/c鼠和SD大鼠;SD大鼠能够产生效价高、特异性好的抗体,线性检测范围为0.01ng/ml～1μg/ml,且SD大鼠具有自身产血量大的特点,具有一定的开发应用价值。 Immunizing Balb/c mouse, Kunming mouse, SD rat respectively with Clenbuterol immunogen whose coupling ratio is 13.2：1, getting the anti-Clenbuterol serum and detecting them with indirect ELISA and indirect competitive ELISA. the results show that the antiserum of Kunming mouse is lower than that of Balb/c mouse and SD rat in the matter of titer, specificity and sensitivity; the antiserum of SD rat has a higher titer and sensitivity, the linear detection scope was 0.01ng/ml-1μg/ml, which has the value of development and application with the feature of big blood volume.

关 键 词： 克喘素 免疫原 多抗血清

领　　域： [生物学]