机构地区: 中国热带农业科学院南亚热带作物研究所
出 处: 《生命科学仪器》 2005年第4期38-40,共3页
摘 要: 用高效液相色谱法测定了几种水果中的维生素C,并研究了不同的色谱条件、不同的提取液对测定结果的影响,结果表明:用0.25%的偏磷酸浸提样品以及作为流动相效果较好,测得Vc的保留时间为4.35,回收率为92%~98%,线性范围0.0001~1.68mg/ml。 Using HPLC to measure the ascorbic acid in fruits with different chromatograph conditions such as different mobile phase, extracting solution. The result shows that 0.025% HPO3 is far better than H3PO4 (pH2.55) solution when acting as mobile phase or extracting solution. That the recover rate between 92% and 98%, the relative coefficient above 0.9994 and the RSD value less than 1.7% proves that the test method by HPLC is suitable.