作　　者： ; ;

机构地区： 中国农业科学院兰州兽医研究所

出　　处： 《畜牧兽医学报》 1995年第2期168-173,共6页

摘　　要： 本文报道了应用十二烷基硫酸钠一聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ），聚丙烯酰胺凝胶等电聚焦（ＩＥＦ）电泳及二维电泳（ＩＥＦ／ＳＤＳ－ＰＡＧＥ）对旋毛虫肌动虫排泄分泌（ＥＳ）抗原和肌幼虫可溶性抗原的分析结果。肌幼虫ＥＳ抗原经ＳＤＳ－ＰＡＧＥ后用考马斯亮蓝染色蛋白质，结果显示１６条蛋白带，分子量范围２１～８０ＫＤ，其中主带９条。ＩＥＦ电泳后分别用ＰＡＳ染多糖、考马斯亮蓝Ｒ－２５０染蛋白质、Ｎｉｌｅ＇ｓ蓝染脂、醋酸ａ－萘酯／坚固蓝染酪酶同工酶，结果肌幼虫ＥＳ抗原分别显示１６，２６、７及０条带；肌幼虫可溶性抗原分别显示２１、３８、４及１１条带。二维电泳后用考马斯亮蓝Ｇ－２５０染色多肤斑点，结果ＥＳ抗原显示多肽斑点６１个；肌幼虫可溶抗原显示１２２个多肽斑点。 The Present paper represents the results of a preliminary analysis of excretory-secretory (ES) antigens and soluble antigens of T. spiralis muscle larvae by sodiumdodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE), isoelectrofocusing (IEF)and two-dimensional electrophoresis. After SDS-PAGE, the Proteins were stained bycoomassie brilliant blue R-250. Tlie ES antigens sliowed 16 bands, with niolecular weightranging between 21-80KDa. After IEF, the sugars were stained by PAS, the lipids byNile's blue, the proteins by comassie blue R-250, the esterase isoenzyines by fastblue. The results showed 16, 7, 26 and O bands in ES antigens and 21, 4, 38 and 11bands in soluble antigens respectively. There existed 61 polypeptide spots in ES antigensand 122 in soluble antigens respectively, which were stained by coomassie blue G一250。Most of these spots were low molecular weight acidic polypeptides less than 67 KDa. The resnIts indicate that the ES and soluble antigens of muscle larvae contain atleast glycoproteins,Iipoproteins, glycolipid proteins,proteins and sugars。

关 键 词： 旋毛虫 排泄分泌抗原 肌幼虫 可溶性 抗原 电泳

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]