作　　者： ; ; (赵永昌）;

机构地区： 云南农科院生物技术研究所

出　　处： 《西南农业学报》 1995年第2期94-100,共7页

摘　　要： 本文以佛罗里达平菇、鲍鱼菇及桃红平菇为材料，研究了食用菌原生质体的单核化、诱变及融合。单核化的原生质体用ｕ．ｖ．照射６０秒时，缺陷型突变率约为０．３％。实验表明，电融合方法所得的融合于主要是非整倍体，所得融合子能明显地改善亲本的酶学特性，这对提高菌种对基质的利用率是非常有意义的。 The monokaryodization,induction and fusion of protoplasts of pleurotus florida,P.cystidiosus and P.salmoneos-tramineus were studied in this paier.The experiment indicated that many protoplasts were monokaryon in two species of Pleurotus. The apparent mutant frequency of protoplasts was 0.3% after they were irradiated 60 seconds by U.V.light. The result also showed that aneuploid had the enzyme characteristics of two parents.

关 键 词： 食用菌 原生质体融合 融合子特性

领　　域： [农业科学] [农业科学]