作　　者： ; ; ;

机构地区： 华南师范大学生命科学学院生物科学系

出　　处： 《实验生物学报》 1995年第4期355-359,共5页

摘　　要： 红光引起黄化绿豆(Phaseolus raditus L.)幼苗下胚轴原生质体积累^(45)Ca^(2+),在光辐照度未达到饱和值前,^(45)Ca^(2+)积累量与光照强度或光照时间呈正相关;远红光可逆转红光引起的^(45)Ca^(2+)积累,^(45)Ca^(2+)积累的多寡取决于最后一次是照射红光还是远红光,表明光敏色素对Ca^(2+)吸收具有调节作用。 Protoplasts from dark grown hypoco-tyl of 64 h-old mung bean (Phaseolus ra-ditus L.) seedlings were maintained at a constant osmotic potential at 20 ±2℃, and fluxes of calcium in protoplasts were analyzed using 45Ca2+. Protoplasts that illuminated with red light (10.5 W·m-2, 3 min) took up 45Ca2+ which was applied before red light treatment. 45Ca2+ uptake by protoplasts increased after 10 min significantly, and was up to maximum (160% as dark control) during 30-60 min after red light irradiation, then followed by a gradual decline. Moreover red light respo-se was in positive correlation with red light intensity and duration of red light plus. The red light effect was photorever-sible by subsequent far-red light (2.5W·m-2, 5 min), usually seen over two red light-far red light cycles. Furthermore, far-red light became less effective in reversing the effect of red light after 5 min dark interval between red light and far-red light. This indicated that phytochrome played an action of regulation on Ca2+ uptake.

关 键 词： 绿豆 黄化绿豆 光敏色素 原生质体 钙 幼苗

领　　域： [农业科学]