作　　者： ; ; ; ; (唐有棋）;

机构地区： 中国科学院化学研究所

出　　处： 《生物物理学报》 1995年第2期149-154,共6页

摘　　要： 采用近红外付上叶拉曼光谱研究了三螺旋ＲＮＡ（ｒＵ）．ｐｏｌｙ（ｒＡ）．ｐｏｌｙ（ｒＵ）在溶液中的构象和在银胶中的表面增强拉曼散射行为。结果表明在溶液中，该三螺旋ＲＮＡ分子中以Ｗａｔｓｏｎ—Ｃｒｉｃｋ碱基配对的两条链处于Ａ－构型，而第二条嘧啶链处于Ｃ２＇－ｅｎｄｏ／ａｎｔｉ构象。在银胶中，该三螺旋ＲＮＡ的表面增强拉曼效应明显。与溶液状态下相比，８３５和８１９ｃｍ－１谱带的出现暗示该三螺旋ＲＮＡ吸附到银胶表面后，该三螺旋ＲＮＡ分子的螺旋结构仍得到保留，且其构象与溶液中的相近。同时该三螺旋ＲＮＡ主要是通过核酸骨架上带负电荷的磷酸基团定位于银胶表面而吸附的。 Near infrared Fourier transform Raman spectroscopy was applied to characterize the triplex RNA poly(rU).poly(rA). poly(rU) in solution and adsorbed on silver colloids. Results revealed that the Watson-Crick base paired strands of the triplex RNA are in the A conformation, While the second ploy(rU) strand has a C2'-endo/anti ribose pucker. Howerver,the triplex RNA M a very strong enhanced effect of Raman signals after adsorbed on colloidal silver surfaces, the surface enhanced factor is up to 2×104. Compared to the results obtained with the triplex RNA in solution, the doublet bands located at 835 and 819cm-1indicate that the belieal structure of the triplex molecules adsorbed on silver surfaces was also Well preserved, and the adsorbed triplexes take on a conformation which is similar to that of the triplex in solution. Moreover, the negatively charged phosphate group of the ribose backbone of the triplex was anchored at the colloidal silver surface.

关 键 词： 三螺旋 表面增强 拉曼光谱 构象

领　　域： [生物学]