作　　者： ; ; ;

机构地区： 南京农业大学植物保护学院植物病理学系

出　　处： 《农业生物技术学报》 1995年第3期65-70,共6页

摘　　要： 以经转座子Ｔｎ５诱变的水稻白叶枯病菌ｄｓｐ基因突变体ＸｏｏＭ３ｌ０４为材料，构建了ｄｓｐ基因探针质粒ｐＶＩＲ３。采用分子杂交法，从基因文库克隆中筛选出１个ｄｓｐ基因克隆ｐＮＡＸ３１１１，含２２ｋｂ的外源ＤＮＡ插入片段，具有４个ＫｐｎＩ酶切位点，其中３．０，２．０ｋｂ两个片段与探针质粒中Ｔｎ５侧翼的ｄｓｐ基因序列同源。以ｐＮＡＸ３ｌｌｌ为探针与Ｘｏｏ日本系统、中国系统及菲律宾系统的菌株，细菌性条斑病菌和菊欧氏杆菌菌株基因组ＤＮＡ及ＪＸＯⅢｈｒｐ基因克隆ｐＮＡＸ３ｌ０３进行同源性分析，结果表明，除与ｐＮＡＸ３ｌ０３和ＪＸＯⅢ基因组ＤＮＡ有同源性外，与其它所有试验菌株均无同源杂交带出现。功能互补分析表明，ｐＮＡＸ３ｌｌｌ能恢复ｄｓｐ基因突变体ＸｏｏＭ３ｌ０４的淀粉酶活性和对水稻的致病性。 A dsp gene probe(pVⅠ R3)including DNA fragment of Tn5 and dsp flankinng sequencesfrom a Tn5一induced rnutant(XooM3l04)of Xanthomnas oryzae pv,oryzae JXOⅢwas constructed.Onedsp gene clone(pNAX3lll)was obtained by screening the genomic library of JXOⅢwith α-32-P-dCTP la-belling pVIR 3 in colony hybridization experiment.pNAX3lll carries 22 kb DNA inserts in which fourKpnl restruction sites were located and two DNA fragmants(2. 0 kb and 3.0 kb)was homolog ous withpVIR3 in southern blot analysis. No homological sequences could be found in all of the tested strains ofXoo races except JXOⅢ, and also in X.oryzae pv. oryzicola, Erwinia chrysanthemi,but the high homol-ogy existed between hrp genes and dsp genes of JXOⅢ.When pNAX3lll was transfered into a dsp genemutant(XooM3l 04),the pathogenlcity to rice and amylase activity of transconjugants was restored assame phnotynes as JxOⅢ.

关 键 词： 水稻 白叶枯病菌 同源性分析

领　　域： [农业科学]