作　　者： ; ; ; ;

机构地区： 华南理工大学生物科学与工程学院

出　　处： 《贵州大学学报（自然科学版）》 2005年第3期301-304,共4页

摘　　要： 2-脱氧葡萄糖是一种酵母细胞壁合成抑制剂,红发夫酵母培养中添加0.0005%-0.002%(w/v)的2-脱氧葡萄糖可使生物量基本保持不变,但能促进虾青素积累,在增加虾青素产量的同时缩短酶法提取的时间。当添加浓度为0.002%时,虾青素含量可提高到对照组的113.7%,若采用胞壁溶解酶对红发夫酵母破壁提取虾青素,作用12h提取率可达94.7%。同时,2-脱氧葡萄糖应在红发夫酵母培养的前36h添加。 The concentration of 2-deoxy-D-glucose in YM medium significantly affected the biomass and astaxanthin content of Phaffia rhodozyma and the extraction of astaxanthin with lytic enzyme. Optimal concentration which led to 13.7% enhancement of astaxanthin content and, more importantly, made extraction of astaxanthin easy, is 0.002% （w/v）. When the yeast was cultivated under this condition, extractability of astaxanthin can reach 94.7% after 12h treatment with lytic enzyme. In addition, 2-deoxy-D-glucose should be added during the previous 36h of culture time of Phaffia rhodozyma.

关 键 词： 脱氧葡萄糖 红发夫酵母 虾青素 胞壁溶解酶

领　　域： [生物学]