作　　者： ; ; ; ; ; (罗勇）;

机构地区： 暨南大学生命科学技术学院化学系

出　　处： 《植物生理与分子生物学学报》 2005年第4期369-373,共5页

摘　　要： 50 mg/L 的硒胁迫下,小球藻中β- 胡萝卜素、类叶黄素、叶绿素(叶绿素a、叶绿素b)的含量先明显增加,之后逐渐下降;而在800 mg/L硒胁迫下,各种光合色素含量均明显下降;小球藻活体细胞在693 nm处出现叶绿素a 的吸收峰,其吸收值在硒胁迫后明显减弱;在室温荧光发射光谱中,700 nm处的发射峰随着硒浓度的增加而显著下降;荧光激发光谱表明:硒胁迫使小球藻的能量传递受阻,传递效率降低;藻体中水溶性蛋白含量随着硒胁迫的增强而下降。等离子体原子发射光谱(ICP-AES)研究结果表明:随着硒浓度增加,藻体中Mg2+、Ca2+、K+ 和Na+ 含量呈明显降低趋势,而培养液中这些离子的浓度则不断增加。 Changes in photosynthetic pigment and protein contents, growth and the spectral characteristics in Chlorella vulgaris in response to selenium stress were investigated. Carotenoids （13-carotene and xanthophylls） and chlorophyll （Chl a and Chl b） contents in cells exposed to Se 50 mg/L increased primarily and decreased afterwards, while photosynthetic pigments in cells exposed to Se 800 mg/L decreased significantly. Chlorophyll （Chl） absorption peak at 693 nm and prominent Chl emission peak at 700 nm, weakened significantly after Se stress. The excitation spectra showed a decrease in excitation energy transfer efficiency in Se-stressed cells. Total soluble protein decreased after Se stress. The changes in total Se, Mg^2＋,Ca^2＋, K^＋ and Na^＋ concentrations in culture medium and cells were also determined by ICP-AESo

关 键 词： 硒胁迫 小球藻 光合色素 光谱性质

领　　域： [生物学]