作　　者： ; ; ; ; ; ; ;

机构地区： 环境与疾病相关基因教育部重点实验室

出　　处： 《中国地方病学杂志》 2005年第4期382-385,共4页

摘　　要： 目的通过补硒对正常鸡胚脑和肝脏中谷胱甘肽过氧化物酶(GPx)和脱碘酶活性的影响,探讨补硒对于正常动物的影响。方法对孵育8d的鸡胚注射不同剂量的甲基硒代半胱氨酸(MSeC),含硒量分别为0、1、5、10、20μg,第20天时测定脑组织和肝脏中硒水平、GPx活性及脱碘酶活性。结果对正常鸡胚补硒,可有效提高脑和肝脏的硒水平及GPx活性,还可提高肝脏Ⅰ型脱碘酶(IDⅠ)活性,但对脑中Ⅱ型脱碘酶(IDⅡ)活性没有影响。补硒量与除脑IDⅡ活性外的其他指标都显著相关(P<0.01)。结论MSeC是一个有效的补硒制剂,对于正常鸡胚,补硒可提高肝脏、脑组织硒水平和肝GPx、IDⅠ活性及脑GPx活性,其补硒效应有随补硒量增加而增强的趋势。 Objective To investigate the effect of selenium supplementation on activities of glutathioneperoxidase(GPx) and iodothyronine deiodinase(ID) in chicken embryonic brain and liver. Methods Normal 8-daychicken embryos were injected with Se-methylselenocysteine (MSeC) of different concentrations (0, 1, 5, 10 or 20μg selenium). Selenium concentration, GPx and iodothyronine deiodinase activities were assayed in 20-dayembryos. Results Suppling MSeC to normal chicken could increase selenium content and GPx activity in bothbrain and liver, and also increase IDⅠ in liver, but could not change IDⅡ activity in brain. MSeC dosage hadsignificant correlations with all the parameters (P < 0.01) except IDⅡ activity. Conclusions MSeC, an effectiveselenium supplement, increases the selenium level and GPx activity in normal chicken embryonic brain and liver,and IDⅠ activity in liver, possibly in a dose-dependent manner.

关 键 词： 硒 甲基硒代米胱氨酸 脱碘酶 谷胱目肽过氧化酶 鸡胚

领　　域： [理学] [理学] [生物学]