作　　者： ; ; ; ; (崔亚利）; (梁万宁）;

机构地区： 中国农业大学动物医学院

出　　处： 《畜牧兽医学报》 2005年第7期690-694,共5页

摘　　要： 用饲喂正常标准兔料的去势雌兔作为对照组,高胆固醇饮食法复制动脉粥样硬化模型。采用βactin做内参照的半定量RTPCR方法,对2组去势雌兔心脏、肝脏组织内雌激素受体α和低密度脂蛋白受体mRNA的表达进行了研究。结果表明:2种受体在兔心脏和肝脏内均有表达;对照组,雌激素受体在心脏、肝脏内的相对表达量为0.43±0.12、0.39±0.20,低密度脂蛋白受体在心脏、肝脏内的相对表达量为0.27±0.05、0.86±0.12,差异显著(P<0.01);模型组,雌激素受体和低密度脂蛋白受体在心脏、肝脏内的相对表达量分别为0.29±0.03、0.27±0.06和0.04±0.01、0.17±0.02。 In present study, atherosclerotic animal model were replicated with high cholesterol feedstuff , and the expressions of estrogen receptor α and low-density lipoprotein receptor mRNA in heart and liver were studied by half quantitive RT-PCR, the rabbits oophorectomized and fed with normal feedstuff were used as control.The results showed:the two sorts of receptors expressed in heart and liver;in control group, the relative expression of ERα mRNA in the heart and liver were 0.43±0.12, 0.39±0.20,the relative expression of low-density lipoprotein receptor mRNA in the heart and liver were 0.27±0.05, 0.86±0.12; The relative expression of estrogen receptor and low-density lipoprotein receptor mRNA in the heart and liver of model rabbits were 0.29±0.03, 0.27±0.06 and 0.04±0.01, 0.17±0.02 respectively.

关 键 词： 雌激素受体 低密度脂蛋白受体 兔 动脉粥样硬化

领　　域： [农业科学] [农业科学] [农业科学]