作　　者： ; ; ;

机构地区： 中山大学化学与化学工程学院

出　　处： 《分析测试学报》 2005年第4期45-47,共3页

摘　　要： 南海海洋真菌Hypoxylonoceanicum(326#)以葡萄糖(10g/L)、蛋白胨(2g/L)、酵母膏(1g/L)、20%的人工海水为培养基,在培养液中添加200mg/L的1,2,3,4-四氢萘,培养7d,对转化产物进行提取,经GC-MS和NMR检测,发现转化产物中有1个主要氧化产物3,4-dihydro-4-hydroxy-1(2H)_naphthalenone,占96.16%。另外3个氧化产物为:3,4-dihydro-1(2H)-naphthalenone,1,2,3,4-tetrahydro-1-naphthalenol和1,2,3,4-tetrahydro-1,2-naph-thalenediol。该微生物催化氧化反应发生在四氢萘的非活性的饱和碳上,显示出海洋真菌Hypoxylonoceanicum(326#)具有独特的生物氧化机制。 1,2,3,4- Tetrahydronaphthalene was biotransformed by marine fungus Hypoxylon oceanicum(326#)fromthe South China Sea.Glucose(10g/L),peptone(2g/L),yeastextract(1g/L),and20%arti-ficial seawater(ASW)were mixed to prepare the liquid medium for the marine fungus.A sterilized substrate,1,2,3,4-tetrahydronaphthalene(200mg/L)was distributed in the culture flasks and fermented for7days.The biotransformation products was successively extracted with EtOAc,and was measured by GC-MS and NMR.The results showed that there were four oxidative products:major product3,4-dihydro-4-hydroxy-1(2H)-naphthalenone(96.16%of the total)and three minor products:3,4-dihydro-1(2H)-naphthalenone,1,2,3,4-tetrahydro-naphthalenol and1,2,3,4-tetrahydro-1,2-naphthalenediol.It was interesting to observe thatthe oxidationreactions occurredatthe non-activatedalicyclic skeleton.This indicatedthat Hypoxylonocean-icumhad the unique bio-oxidation mechanism.

关 键 词： 海洋真菌 生物转化 四氢萘 气相色谱 质谱 核磁共振

领　　域： [理学] [理学] [理学] [理学]