机构地区: 中山大学生命科学学院生物系
出 处: 《西南林学院学报》 1989年第1期8-13,共6页
摘 要: 干藏的珙桐种子存在酸性磷酸酶,经层积1年后,该酶的同工酶及酶活性均有所增加。随着种子的萌发,同工酶谱带也增加,表明酸性磷酸酶与打破珙桐种子休眠和萌发过程有密切关系。层积1年后的珙桐种子只有少部分能打破休眠,大部分仍处于休眠状态,不能萌发。对这些难萌发的种子,用50ppm 6—BA处理有促进萌发的作用;用100ppm GA_2和50ppm 6—BA处理,其酸性磷酸酶同工酶谱带显著增加。用100μM 5—Fu、100μM CH和20ppm ABA处理,则抑制珙桐种子的萌发,也抑制酸性磷酸酶同工酶的合成。 Acid phosphatase was found in dried Davidia involucrata seeds. After stratification for one year, the isospecies and activity of acid phosphatase in seeds were increased. During germination of seeds, the increases of enzyme were also found. It is clear that acid phosphatase is related to dormancy and germination of dovetree seeds. After stratification for one year, only some,seeds could germinate while the majority were still dormant. These dormant seeds could be hastened to germinate with the increase of isophosphatases by use of 50 ppm 6-BA. Seeds treatcd with 100 ppm GA_3 and 50 ppm 6-BA had more bands on zymogram of isophosphatases. Treatment of 100μM 5-Fu, 100ppm CH and 200 ppm ABA dcpressed germination of seeds and inhibited synthesis of isophosphatase.