作　　者： ; ; ; ; ;

机构地区： 中山大学生命科学学院水生经济动物繁殖营养和病害控制国家重点学科专业实验室

出　　处： 《海洋学报》 2005年第3期147-153,共7页

摘　　要： 研究了生长抑素抑制剂半胱胺盐酸盐(CSH)和促黄体素释放激素类似物(LHRH A)对黄鳍鲷生长激素(GH)分泌的影响.黄鳍鲷垂体GH含量在单独注射不同剂量CSH(25,50,100μg/gb.w)后2h均显著提高,注射后24h恢复正常.黄鳍鲷血清中GH水平在注射CSH(100μg/gb.w)后2h和CSH(25,50μg/gb.w)后6h均明显提高.单独注射LHRH A(0.1μg/gb.w)后2h黄鳍鲷垂体GH含量和血清中GH水平均明显提高.注射CSH和LHRH A后6h垂体GH含量明显高于单独注射CSH或LHRH A,而血清GH水平在注射后12h明显高于单独注射CSH或LHRH A.结果表明CSH和LHRH A都能明显刺激黄鳍鲷垂体合成和释放GH,并且与CSH作用存在剂量依存关系,CSH和LHRH A联合注射比单独注射作用更明显,作用的时间更持久. Effects of cysteamine hydrochloride (a somatostatin-inhibiting agent,CSH)and luteinizing hormone-releasing hormone analog on growth hormone secretion in Sparus latus were investigated by i.p. injection.The pituitary GH contents of Sparus latus were significantly increased 2 h post i.p. injection of CSH with doses of 25,50 and 100 μg/gb.w,and returned to control levels 24 h post injection. The serum GH levels of Sparus latus were significantly increased 2 h post injection of CSH with dose of 100 μg/gb.w,and 6 h post injection of CSH with doses of 25 and 50μg/gb.w; LHRH-A(0.1 μg/gb.w) significantly increased pituitary GH contents and serum GH levels 2 h post injection. Combining injection of LHRH-A and CSH significantly increased pituitary GH contents 6 h post injection as compared with CSH or LHRH-A alone,and the serum GH levels were significantly increased 12 h post injection as compared with CSH or LHRH-A alone.These results indicate that both CSH and LHRH-A could stimulate the synthesis and release of GH in Sparus latus, and the effects of CSH were dose dependent. The combined effects of CSH and LHRH-A were significantly higher than those of CSH or LHRH-A alone.

关 键 词： 半胱胺盐酸盐 促黄体素释放激素类似物 黄鳍鲷 生长激素

领　　域： [生物学]