作　　者： ; ; ; ; ; ;

机构地区： 东北农业大学农学院大豆研究所

出　　处： 《大豆科学》 2005年第2期90-94,共5页

摘　　要： 实验构建了CaMV35S启动子控制下的大豆抗病相关基因SR1的正反义植物双元表达载体pBISR1(+)和pBISR1(-)。通过根癌农杆菌叶盘转化法,将正义和反义SR1 基因导入烟草Ha vana 425,经卡那霉素筛选,获得了抗性植株。经PCR和PCR-Southern印迹分析,证明抗性植株中整和了SR1基因,RT-PCR分析进一步表明正义和反义基因皆能转录为完整的mRNA,经疫霉根腐接种及抗病性鉴定表明,转反义基因株系和未转基因株系均轻微感病,而转正义基因株系始终没有出现感病症状。 Two plant expression vectors carrying sense or antisense soybean resistance-related gene SR1 under the regulation of cauliflower mosaic virus 35s promoter was constructed. Leaf segments of tobacco Havana 425 were infected by Agrobacterium tumefaciens LBA4404 with pBISR1(+) or pBISR1(-), from which kanamycin resistant plants were obtained. PCR and PCR-Southern analysis proved that the SR1 gene was integrated into the genomes of the tobacco plants, and RT-PCR analysis proved that sense or antisense gene was transcripted into a complete mRNA. The disease resistance assay showed that plants with antisense gene and control plants were slightly susceptible, and plants with sense gene were not susceptible.

关 键 词： 抗病相关基因 反义植物表达载体 遗传转化 构建 大豆 双元表达载体 启动子 抗性植株 反义基因 分析 抗病性鉴定 基因导入 卡那霉素 正义基因 转化法 株系 转基因 感病 根癌 烟草 接种 根腐 疫霉

领　　域： [农业科学] [农业科学] [农业科学]