机构地区: 广东省农业科学院
出 处: 《核农学报》 2005年第3期195-197,213,共4页
摘 要: 本文对产纤维素酶的木霉菌孢子和斜面培养菌体搭载中国第18颗返回式卫星后,菌落形态和产酶能力的变化进行了研究。实验结果表明,搭载菌株的菌落形态发生变异,菌株的生长周期出现了缩短或延长的现象。对搭载的木霉菌株进行筛选,获得3株产酶能力大幅度提高的优良突变菌株,产酶能力提高了50%以上,且性状遗传稳定。 The slant mycelia cultured with or without mutagenic agent diethyl sulfate(DS) and spores of Trichoderma reesei were loaded in the 18^(th) returning satellite. Systematical screening showed that the life cycle and morphology of some strains had changed after space flight. After selection and domestication, 3 mutant strains with high cellulose enzyme activity were isolated. The cellulose enzyme productivities of the mutants were significantly increased more than 50%, and the mutant were generically stable.
关 键 词: 空间诱变效应 产酶能力 返回式卫星 斜面培养 霉菌孢子 纤维素酶 菌落形态 形态发生 生长周期 木霉菌株 突变菌株 能力提高 遗传稳定 搭载 大幅度