作　　者： ; ; ; ;

机构地区： 厦门大学

出　　处： 《寄生虫与医学昆虫学报》 2005年第2期71-76,共6页

摘　　要： 本文对普遍寄生于家畜及野生动物胰脏并引起胰吸虫病的几种阔盘属(Eurytrema)吸虫:腔阔盘吸虫(Eurytremacoelomaticum)、胰阔盘吸虫(Eurytremapancreaticun)、枝睾阔盘吸虫(Eurytremacladorchis)及福建阔盘吸虫(Eurytremafukienensis)的系统发生关系进行比较研究。分别提取寄生于不同种类宿主的这4种胰吸虫的基因组DNA ,利用随机引物进行PCR扩增,从80个随机引物中筛选出重复性好、谱带清晰并呈多态性的引物31个,共扩增出30 3条DNA带。根据重复性好、谱带清晰并呈多态性的引物扩增的结果计算遗传距离,根据遗传距离利用UPGMA方法进行聚类分析,构建出阔盘属几种吸虫的系统发生树,分子系统发生与形态学分类一致。 This paper reported the phylogenetic relationship of species from Eurytrema.Genomic DNA of the six kinds of Eurytrema was extracted after identification and quantitative analysis.The RAPD-PCR was performed and screen those primers which could amplify clear and repeating bands.In 80 arbitrary primers,31 of them could amplify clear and repeating bands.A total of 303 fragments were obtained.The amplifying results was analysis by unweighted pair-group method with arithmetical averages(UPGMA).The phylogenetic tree by UPGMA are reconstructed based on genetic distance data,which is paralleled with the morphological classification.

关 键 词： 野生动物 家养动物 阔盘属吸虫 系统发育 寄生虫

领　　域： [农业科学] [农业科学] [农业科学]