机构地区: 复旦大学生命科学学院
出 处: 《Acta Genetica Sinica》 2005年第6期600-607,共8页
摘 要: 利用RACE PCR方法从荠菜(Capsellabursa pastoris)中克隆到了新的LOS2全长基因(Cblos2)。序列分析表明,该基因的全长cDNA为1694bp,拥有一个由444个氨基酸组成的开放读码框,预测的CbLOS2蛋白包括一个烯醇酶N 端结构域、烯醇酶结构域以及与拟南芥的LOS2高度保守的DNA结合域和基因功能抑制域。生物信息学分析表明,Cblos2与LOS2极为相似。冷胁迫适应实验表明,Cblos2基因在荠菜中组成性表达,且其表达与胁迫适应过程密切相关。该研究表明Cblos2是具双重功能的植物烯醇酶基因家族的新成员。 A new LOS2 gene was cloned from C.bursa pastoris by rapid amplification of cDNA ends (RACE).The full length cDNA of C.bursa pastoris LOS2 gene (designated as Cblos2 ) was 1 694 bp containing a 1 332 bp open reading frame (ORF) encoding a 444 amino acid protein.The predicted CbLOS2 protein contained enolase N domain,enolase domain,conserved putative DNA binding and repression domains like LOS2 from A.thaliana .Bioinformatic analysis indicated that CbLOS2 had similarity with other enolase proteins.Cold acclimation assay revealed that Cblos2 expressed constitutively in C.bursa pastoris and was involved in the cold acclimation process,implying CbLOS2 was a bi functional enolase.
领 域: [生物学]