作　　者： ; ; ; ; ; ; ;

机构地区： 淮阴师范学院生命科学学院

出　　处： 《微生物学通报》 2005年第3期91-94,共4页

摘　　要： 为提高虫草素和腺苷的代谢量,用高效液相色谱法作检测手段,以二者含量为检测指标,对液体培养的氮源种类、氮源水平、碳源水平、动态变化、培养体系的虫草素及腺苷含量和总量进行研究,结果表明:不仅蚕蛹粉,豆粕和豆粉也是很好的氮源;培养液以3%氮源、4%碳源为佳;振荡培养8d,培养液中虫草素总量是菌丝体中虫草素总量的6倍多;振荡培养7～9d,可使每瓶培养物的虫草素和腺苷总量达到10mg以上. In order to improve the metabolism yield of cordycepin and adenosine,we studied nitrogen sources ,the levels of nitrogen sources and carbon sources,dynamic development in liquid culture ,as well as the total yield of cordycepin and adenosine in culture system through determining the contents of cordycepin and adenosine by HPLC .The results are as follows: not only animal protein but also some plant protein is very good nitrogen sources; in culture solution the suitable levels of nitrogen sources and carbon sources is respectively 3% and 4%; the content of adenosine in culture solution is very low, while it is quite high in mycelia; it is much higher that the total yield of cordycepin in culture solution than in mycelia; when the culture system is oscillated and cultured after 7~9 days, the total yield of cordycepin and adenosine are both high.

关 键 词： 液体培养 蛹虫草 虫草素 腺苷 代谢量 培养条件 高效液相色谱法

领　　域： [轻工技术与工程] [化学工程] [生物学]