作　　者： ; ;

机构地区： 中国医学科学院北京协和医学院

出　　处： 《中国医学科学院学报》 1994年第2期104-108,共5页

摘　　要： 采用培养板钻孔后覆以Ｆｏｒｍｖａｒ膜代替金网为支撑物培养细胞，并改良了抽提条件及观察条件，对人红白血病细胞Ｋ５６２的核基质－中间纤维体系结构进行了观察。结果显示，Ｋ５６２细胞核基质为粗细不等、分布不规则的纤维交织而成的网状结构，胞质中的中间纤维主要呈放射状分布，并借网板状的核纤层与核基质相连。本研究所采用的整装电镜制样方法与同类方法相比较，材料来源方便、经济，盲目性小，人工假象少，成功率较高，所得的Ｋ５６２细胞核基质－中间纤维图像清晰，为研究哺乳类红系细胞终末分化期的自然排核机理提供了有效手段，并为进一步探讨核基质－中间纤维体系在肿瘤细胞恶性调控中所起的作用奠定了形态学基础。 We applied culture plates with bored holes covered with FORMVAR and coated with carbon,to replace the gold grids used in conventional whole mount TEM study.Human erythroleukemia cell line K562 cells plated on the plate surface were extracted using a modified protocol to study scaffolds of nuclear matrix(NM)and intermediate filament(IF).The NM of the K562 cells was found to be composed of interweaving filaments of different diameters,while the cytoplasmic IF were mainly distributed in a radialized pattern.Compared with other techniques of whole mount TEM sample preparations,this one is much more practical and economical,yielding clear NM-IF structures with few artifacts.Study of NM-IF scaffolds in K562 cells might provide a basis for further elucidation of the involvement of NM-IF in the denucleation of mammalian erythroblasts.

关 键 词： 整装电镜 核基质 细胞

领　　域： [生物学]