作　　者： ; ; ; ; ; ;

机构地区： 中山大学化学与化学工程学院

出　　处： 《应用化学》 2005年第6期581-585,共5页

摘　　要： 建立了毛细管电泳高频电导法同时测定阿莫西林和克拉维酸钾片中二组分的方法。对电泳介质的种类、浓度及分离电压和进样量等因素进行了优化。在1.0mmol/LNa2HPO4+0.50mmol/LNaH2PO4(pH=8.0)、分离电压20.0kV的条件下,可在6.0min内实现阿莫西林和克拉维酸钾的分离检测。线性范围分别为3.0×10-6～160.0×10-6g/mL和2.0×10-6～150.0×10-6g/mL,检出限分别为1.5×10-6g/mL和8.0×10-5g/mL,回收率分别达97.0%～101.0%和97.7%～98.7%。 A method to determine simultaneously two active components in amoxicillin and potassium clavulanate tablets by capillary electrophoresis with high frequency conductivity detection was established. The key effective factors for the separation and determination were studied and the best analysis conditions were obtained. The amoxicillin and potassium clavulanate could be optimally separated and detected in the running medium of 1.0 mmol/L Na_ 2HPO_ 4+0.5 mmol/L NaH_ 2PO_ 4(pH=8.0) at a separation voltage of 20.0 kV. Under the optimal conditions, the sample separation and detection only take 6 min, the determined peaks have good shapes, and the linear concentration range is from 3.0×10 -6 g/mL to 160.0× 10 -6 g/mL for amoxi- cillin(r=0.997) and from 2.0×10 -6 g/mL to 150.0×10 -6 g/mL for potassium clavulanate(r=0.998). The detection limits are 1.5 ×10 -6 g/mL and 8.0×10 -6 g/mL, and the recovery rates are 97.0%～101.0% and 97.7%～98.7% for amoxicillin and potassium clavulanate, respectively.

关 键 词： 毛细管电泳 高频电导检测 非接触式电导检测 阿莫西林 克拉维酸

领　　域： [理学] [理学]