作　　者： ; ; ; ; ;

机构地区： 北京林业大学生物科学与技术学院

出　　处： 《北京林业大学学报》 2005年第3期72-75,共4页

摘　　要： 该文采用白色链霉菌为实验菌种,在不同诱导产酶培养基上经过2 40h的振荡培养,探索其产酶时程规律.结果表明,不同的诱导底物诱导产生的最大木聚糖酶活不等,其中木粉的诱导效果好于纯木聚糖,复合底物的诱导效果好于单一底物.该菌在筛选出的最佳诱导产酶培养基上培养14 4h后达到产酶高峰,粗酶液酶活可达到45 66U mL .对该酶进行高温及碱性处理的结果表明,它具有较好的耐热耐碱性,在pH 7 0下反应表现最高活性,同时在90℃下保温3 0min后酶活残留达83 46% Time course of xylanase' production was studied by culturing Strepotmyces albus in different substrates for 240 h. The results show that xylanase demonstrates different activities in different substrates. For induction of xylanase, wood powder is better than xylan, while a compound substrate is better than a single one. After Strepotmyces albus is cultured for 144 h in the optimal enzyme_induction medium, the production of xylanase culminates, with a maximum enzyme activity of 45 66 U/mL. The results show that the xylanase from Strepotmyces albus has the characteristics of both thermotolerance and alkali_tolerance, showing that 83 46% of its maximal activity remains after a treatment for 30 min at 90℃, and the highest activity in pH 7 0.

关 键 词： 白色链霉菌 木聚糖酶 耐热性 耐碱性

领　　域： [生物学] [环境科学与工程]